@article{10.1371/journal.pone.0108128, author = {Pyne, , Derek G. AND Liu, , Jun AND Abdelgawad, , Mohamed AND Sun, , Yu}, journal = {PLoS ONE}, publisher = {Public Library of Science}, title = {Digital Microfluidic Processing of Mammalian Embryos for Vitrification}, year = {2014}, month = {09}, volume = {9}, url = {http://dx.doi.org/10.1371%2Fjournal.pone.0108128}, pages = {e108128}, abstract = {
Cryopreservation is a key technology in biology and clinical practice. This paper presents a digital microfluidic device that automates sample preparation for mammalian embryo vitrification. Individual micro droplets manipulated on the microfluidic device were used as micro-vessels to transport a single mouse embryo through a complete vitrification procedure. Advantages of this approach, compared to manual operation and channel-based microfluidic vitrification, include automated operation, cryoprotectant concentration gradient generation, and feasibility of loading and retrieval of embryos.
}, number = {9}, doi = {10.1371/journal.pone.0108128} }