TY - JOUR T1 - On-chip sample preparation for complete blood count from raw blood A1 - Nguyen, John A1 - Wei, Yuan A1 - Zheng, Yi A1 - Wang, Chen A1 - Sun, Yu Y1 - 2015 SP - 1533 EP - 1544 JF - Lab on a Chip JO - Lab Chip VL - 15 IS - 6 PB - The Royal Society of Chemistry SN - 1473-0197 DO - 10.1039/C4LC01251H M3 - 10.1039/C4LC01251H UR - http://dx.doi.org/10.1039/C4LC01251H N2 - This paper describes a monolithic microfluidic device capable of on-chip sample preparation for both RBC and WBC measurements from whole blood. For the first time, on-chip sample processing (e.g. dilution, lysis, and filtration) and downstream single cell measurement were fully integrated to enable sample preparation and single cell analysis from whole blood on a single device. The device consists of two parallel sub-systems that perform sample processing and electrical measurements for measuring RBC and WBC parameters. The system provides a modular environment capable of handling solutions of various viscosities by adjusting the length of channels and precisely controlling mixing ratios, and features a new 'offset' filter configuration for increased duration of device operation. RBC concentration, mean corpuscular volume (MCV), cell distribution width, WBC concentration and differential are determined by electrical impedance measurement. Experimental characterization of over 100 000 cells from 10 patient blood samples validated the system's capability for performing on-chip raw blood processing and measurement. ER -