TY - JOUR M1 - Copyright 2005, IEE TI - Manipulation of cells using an ultrasonic pressure field AD - Center of Mech., Swiss Fed. Inst. of Technol., Zurich, Switzerland JO - Ultrasound in Medicine and Biology T3 - Ultrasound Med. Biol. (USA) A1 - Haake, A. A1 - Neild, A. A1 - Deok-Ho Kim A1 - Jong-Eun Ihm A1 - Yu Sun A1 - Dual, J. A1 - Byeong-Kwon Ju VL - 31 IS - 6 PY - 2005/06/ U1 - 8674251 SP - 857 EP - 64 SN - 0301-5629 CY - USA PB - Elsevier N2 - A novel device is described that generates an ultrasonic force field in a fluid layer. The force field arises because of the acoustic radiation force, a second order effect, generated as an ultrasonic wave interacts with a suspended particle. This force field can be used to manipulate objects in the fluid layer trapped between this device and an arbitrary surface, in this case, a flat object slide. The device is shown to be capable of positioning and, in doing so, concentrating human cells to predictable locations. Mesenchymal and HeLa cells were used. Critically, the forces required to do this can be generated by ultrasonic pressure fields that do not affect the viability of the cells. The viability has been assessed using trypan blue dye. The device used consists of a 14 mm square glass plate that is excited by at least one of four piezotransducers attached to the edges. The resulting ultrasonic force field and, importantly, the location of the minima in the force potential at which the cells are collected, has been calculated analytically. (E-mail: adrian.neild@imes.mavt.ethz.ch) [All rights reserved Elsevier] KW - biological effects of ultraviolet radiation KW - biomedical equipment KW - cellular effects of radiation KW - piezoelectric transducers KW - surface acoustic waves KW - suspensions KW - ultrasonic transducers U2 - cell manipulation U2 - ultrasonic pressure field U2 - ultrasonic force field U2 - ultrasonic wave interaction U2 - suspended particle U2 - fluid layer U2 - flat object slide U2 - mesenchymal cells U2 - HeLa cells U2 - cell viability U2 - trypan blue dye U2 - square glass plate U2 - piezotransducers U2 - standing wave U2 - Lamb wave U2 - 14 mm L2 - http://dx.doi.org/10.1016/j.ultrasmedbio.2005.03.004 ER -